|
KMID : 0624620180510070338
|
|
BMB Reports
2018 Volume.51 No. 7 p.338 ~ p.343
|
|
|
Investigation of functional roles of transcription termination factor-1 (TTF-I) in HIV-1 replication
|
|
Park Seong-Hyun
Yu Kyung-Lee
Jung Yu-Mi
Lee Seong-Deok
Kim Min-Jeong
You Ji-Chang
|
|
|
Abstract
|
|
|
|
Transcription termination factor-1 (TTF-I) is an RNA polymerase 1-mediated transcription terminator and consisting of a C-terminal DNA-binding domain, central domain, and N-terminal regulatory domain. This protein binds to a so-called ‘Sal box’ composed of an 11-base pair motif. The interaction of TTF-I with the ‘Sal box’ is important for many cellular events, including efficient termination of RNA polymerase-1 activity involved in pre-rRNA synthesis and formation of a chromatin loop. To further understand the role of TTF-I in human immunodeficiency virus (HIV)-I virus production, we generated various TTF-I mutant forms. Through a series of studies of the over-expression of TTF-I and its derivatives along with co-transfection with either proviral DNA or HIV-I long terminal repeat (LTR)-driven reporter vectors, we determined that wild-type TTF-I downregulates HIV-I LTR activity and virus production, while the TTF-I Myb-like domain alone upregulated virus production, suggesting that wild-type TTF-I inhibits virus production and trans-activation of the LTR sequence; the Myb-like domain of TTF-I increased virus production and trans-activated LTR activity.
|
|
|
KEYWORD
|
|
|
c-Myb, Gene Regulation, HIV-1, HIV-1 Long Terminal Repeat, TTF-I, Virus Latency, Virus Replication
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|